Electron density modifications

• Map concepts
  • Molecular envelopes
  • Crystal cell generation
  • Maps: enevelopes, density filled regions
• Solvent flattening
  • The real space procedure
  • The reciprocal space (fast) procedure
  • The score map histogram
  • Filling holes and removeing clouds
  • Geneartion of the unit cell
  • Flattening and flipping of the solvent region
  • Fourier transformation of the solvent flattened map.
  • Solvent flattening procedure based on a skeletonized map
• Generating molecular envelopes
  • Filling holes and removing lonely clouds
  • Avoiding mask overlap(s) in a unit cell
  • Using secondary structure elements (helices)
  • Exploiting map skeletons
  • Procedures based on map statistics
  • Placing atoms on a 10 grid lattice
• Electron density averaging
  • Averaging of electron density: 2 molecules
  • Averaging density between 2 different crystal forms
  • Averaging: yeast proteasome schell script
• Local histogram matching
• Phase combination